Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium   总被引：1，自引：0，他引：1  

Yudkovski Y  Rogowska-Wrzesinska A  Yankelevich I  Shefer E  Herut B  Tom M 《Marine environmental research》2008,65(5):427-436

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Improving debris flow monitoring in Taiwan by using high-resolution rainfall products from QPESUMS   总被引：2，自引：2，他引：2  

Chien-Yuan Chen  Lee-Yao Lin  Fan-Chieh Yu  Ching-Sheng Lee  Chun-Chieh Tseng  An-Hsiang Wang  Kei-Wai Cheung 《Natural Hazards》2007,40(2):447-461

Debris flow is commonly initiated by torrential rain and its triggering is correlated to the hydrological, geological, and geomorphic conditions on site. In spite of the important effects of geology and topography, rainfall characteristic is the main external triggering factor to debris flow and is a predominant parameter for real-time monitoring. Due to the scarcity of sufficient spatial ground-based rainfall data in hill areas, quantitative precipitation estimation using remote-sensing techniques such as radar and satellite is needed for debris flow pre-warning. The QPESUMS (Quantitative Precipitation Estimation and Segregation Using Multiple Sensors) system was acquired to retrieve spatial rainfall data during the rainfall period from June 30 to July 6 in 2004 when Typhoon Mindulle and southwesterly flow struck Taiwan. The retrieved data were used for setting up the debris flow monitoring algorithm. With the aid of multiple platforms of meteorological observations, a rainfall threshold isohyet in a pilot area was mapped for debris flow monitoring. The rainfall monitoring algorithm based on QPESUMS provides more detailed information than the limited number of ground-based rainfall stations for interpreting the spatial distributions of rainfall events, and therefore is more suitable for debris-flow monitoring.  相似文献   

新一代天气雷达站网实时运行监控系统的设计与开发     

蒲晓勇  蔡宏  唐俊  秦建峰 《湖北气象》2007,26(2):179-183

新一代天气雷达站网实时监控系统运用了现代计算机网络、工业控制、数据远程传输、远程控制等多项技术,充分利用新一代天气雷达自身系统监控、重要参数定标、检测等功能进行实时监控系统的设计开发,实现了站网内雷达运行状态、定标及报警等相关信息的实时监控,对全面掌握分中心责任范围内各雷达站的运行状况,提高技术保障系统的快速反应能力,以及全面、快速、有效地进行新一代天气雷达系统设备的技术保障具有重要意义。该文主要概述了新一代天气雷达站网实时监控系统的主要功能、系统结构及关键技术的实现方法。  相似文献   

REAL-TIME FILTERING OF DATA FROM MOBILE,PASSIVE REMOTE INFRARED SENSORS WITH PRINCIPAL COMPONENT MODELS OF BACKGROUND     

S.D.BROWN Department of Chemistry  University of Delaware  Newark  DE  U.S.A. 《地理学报(英文版)》1991,(3)

Real-time monitoring of pollutant levels from a mobile measuring platform requires fast,flexible dataanalysis methods.This paper reports a method for rapid analysis of passive remotely sensed infrared datawith the aid of a Kalman filter.The background spectra produced by emission from the atmosphere aremodelled at the start of the data collection sequence with a simple principal components model obtainedby eigenanalysis of the initial‘blank’data taken with the spectrometer.The species of interest areincluded in the state space model by a separate measurement of their infrared spectra.It is demonstratedthat for best filter performance in detecting the simulated pollutant species SF_6 in the atmosphere,a filtermodel with two principal components describing the emission background works best.The filter‘maps’of SF_6 closely follow the integrated spectral intensities measured after removal of suitable backgrounds.  相似文献   

RECENT DEVELOPMENTS IN MULTIVARIATE CALIBRATION   总被引：1，自引：0，他引：1  

B.R.KOWALSKI M.B.SEASHOLTZ Laboratory for Chemometrics  BG-  University of Washington  Seattle  WA  U.S.A. 《地理学报(英文版)》1991,(3)

With the goal of understanding global chemical processes,environmental chemists have some of the mostcomplex sample analysis problems.Multivariate calibration is a tool that can be applied successfully inmany situations where traditional univariate analyses cannot.The purpose of this paper is to reviewmultivariate calibration,with an emphasis being placed on the developments in recent years.The inverseand classical models are discussed briefly,with the main emphasis on the biased calibration methods.Principal component regression(PCR)and partial least squares(PLS)are discussed,along with methodsfor quantitative and qualitative validation of the calibration models.Non-linear PCR,non-linear PLSand locally weighted regression are presented as calibration methods for non-linear data.Finally,calibration techniques using a matrix of data per sample(second-order calibration)are discussed briefly.  相似文献   

Averaging Intervals For Spectral Analysis Of Nonstationary Turbulence     

George Treviño  Edgar L. Andreas 《Boundary-Layer Meteorology》2000,95(2):231-247

We formulate a method for determining the smallest time interval Tover which a turbulence time series can be averaged to decompose it intoinstantaneous mean and random components. From the random part the method defines the optimal interval (or averaging window) AW over which this part should be averaged to obtain the instantaneous spectrum. Both T and AW vary randomly with time and depend on physical properties of the turbulence. T also depends on the accuracy of the measurements and is thus independent of AW. Interesting features of the method are its real-time capability and the non-equality between AW and T.  相似文献   

一个紧密结构地震台网中心核心数据处理系统   总被引：1，自引：1，他引：0  

王洪体  陈阳  刘明辉  彭朝勇  杜则澄 《地震》2007,27(3):50-56

介绍一个基于UNIX类操作系统的紧密结构地震台网中心核心数据处理系统。 该系统适用于复杂结构的数字地震台网中心, 支持以比较低的成本, 可靠地完成地震台网中心的核心任务, 可以接收来自串口和网络的实时地震波形数据, 支持虚拟数字地震台网组网要求的节点数据服务功能等。 该系统的数据服务协议具有实时性高、 通讯可靠、 适应范围广等特点, 可以满足包括地震预警在内的所有实时地震数据应用的需要。 系统结构紧凑, 自动化程度高, 安全, 稳定, 系统伸缩性大, 可以满足台站数目不大于100个的数字地震台网中心的需要, 同时也可以作为大型数字地震台网中心的处理节点, 构成大型数字地震台网中心的数据处理系统。  相似文献   

固定标本的DNA提取及PCR扩增   总被引：4，自引：1，他引：3  

高天翔  张秀梅  曾晓起  渡边精一 《中国海洋大学学报(自然科学版)》2000,30(2):244-248

报道福尔马林、酒精固定的日本绒螯蟹标本的 DNA提取及 PCR扩增 ,并与冷冻、煮沸标本进行了比较。结果表明 ,福尔马林固定标本与酒精固定标本一样可以提取 DNA,加入的蛋白酶 K量越多 ,DNA的回收率越高。以提取的 DNA为模板 ,进行了线粒体 DNA12 S r RNA和 D- loop基因片段的 PCR扩增 ,经琼脂糖凝胶电泳 PCR扩增产物后均得到了与期待的碱基长度相一致的清晰谱带。  相似文献   

霸王基因组RAPD优化条件的建立   总被引：9，自引：4，他引：5  

郝瑞文  景建洲  李振勇  贾敬芬 《中国沙漠》2006,26(2):286-290

实验以霸王20 d龄无菌籽苗为材料,使用CTAB法提取其基因组DNA,进行RAPD条件优化分析。通过单因子实验分别研究了Mg2+浓度、dNTPS浓度、Taq酶的浓度、引物浓度和模板DNA浓度对RAPD反应的影响,即在25 μL总反应体系中,Mg2+的适宜浓度为1.5~2.5 mmol\5L-1、dNTPs的适宜浓度为0.2~0.3 mmol\5L-1、随机引物的浓度以1.2 μmol\5L-1为宜、Taq酶的用量以2.0 U为佳、模板DNA的最适用量为50 ng。本研究的PCR扩增程序为94℃预变性8 min, 94℃变性1 min, 37℃退火1 min, 72℃延伸2 min,设40个循环,最后72℃保温6 min。  相似文献   

链状亚历山大藻组蛋白H3基因克隆与生长过程中的表达分析     

刘昊昕  Sadaf Riaz  刘源  Anam Khurshid  张巍  隋正红 《海洋科学》2019,43(11):19-27

链状亚历山大藻(Alexandrium catenella)是一种典型的产毒赤潮甲藻。甲藻曾被认为没有组蛋白,但近年来在多种甲藻中检测到全部四个核心组蛋白的活跃转录,而目前对甲藻中组蛋白表达模式与具体功能还缺乏深入的研究。本文报道了链状亚历山大藻组蛋白H3变体之一H3.c的全长ORF序列的克隆和分析;分析了链状亚历山大藻生长过程中组蛋白H3在基因和蛋白水平的表达。目前研究发现的链状亚历山大藻H3变体共三个,其中H3.b在N末端序列与其他物种差异最大,为链状亚历山大藻特有的H3变体。对数生长期的组蛋白H3在基因与蛋白水平上均活跃表达,但表达量并不会随着爆发性增长而显著变化,其中H3.b的基因表达在对数末期呈现上调;培养至衰亡期,各H3变体表达均下调,尤其在蛋白水平上呈现明显衰减。结合前期研究,本文认为链状亚历山大藻三个H3均为复制非依赖型(RI)变体,一些变体可响应伴随藻不断生长而逐渐增强的细胞密度等复杂胁迫,推测其参与某些表观遗传修饰,调控生长进程;而衰亡期基于H3的表观遗传调控受到抑制。  相似文献